Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8513958 | Journal of Pharmaceutical Sciences | 2017 | 30 Pages |
Abstract
To understand interaction of drugs with the prostaglandin transporter OATP2A1/SLCO2A1 that regulates disposition of prostaglandins, we explored the impact of 636 drugs in an FDA-approved drug library on 6-carboxyfluorescein (6-CF) uptake by OATP2A1-expressing HEK293 cells (HEK/2A1). Fifty-one and 10 drugs were found to inhibit and enhance 6-CF uptake by more than 50%, respectively. Effect of the 51 drugs on 6-CF uptake was positively correlated with that on PGE2 uptake (r = 0.64, p < 0.001). Among those, 5 drugs not structurally related to prostaglandins, suramin, pranlukast, zafirlukast, olmesartan medoxomil, and losartan potassium, exhibited more than 90% PGE2 uptake inhibition. Inhibitory affinity of suramin to OATP2A1 was the highest (IC50,2A1 of 0.17 μM), and its IC50 values to MRP4-mediated PGE2 transport (IC50,MRP4) and PGE2 synthesis in human U-937 cells treated with phorbol 12-myristate 13-acetate (IC50,Syn) were 73.6 and 336.7 times higher than IC50,2A1, respectively. Moreover, structure-activity relationship study in 29 nonsteroidal anti-inflammatory drugs contained in the library displayed inhibitory activities of anthranilic acid derivatives, but enhancing effects of propionic acid derivatives. These results demonstrate that suramin is a potent selective inhibitor of OATP2A1, providing a comprehensive information about drugs in clinical use that interact with OATP2A1.
Keywords
INHNSAID6-CFARBphorbol 12-myristate 13-acetateSTI6-carboxyfluoresceinPMADrug effectsInhibitorStimulatorOrganic anion-transporting polypeptide transportersTransportnonsteroidal anti-inflammatory drugStructure-activity relationship (SAR)PharmacodynamicsPharmacokineticsAngiotensin receptor blockercysteinyl leukotriene receptor
Related Topics
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Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Shunsuke Kamo, Takeo Nakanishi, Rika Aotani, Yoshinobu Nakamura, Tomoka Gose, Ikumi Tamai,