Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8514312 | Journal of Pharmaceutical Sciences | 2017 | 5 Pages |
Abstract
A relationship between the chenodeoxycholate (CDC) monomer concentration and the total concentration of CDC was established using a kinetic dialysis technique. Meanwhile, the sizes of the formed simple CDC micelles were measured by a quasielastic light-scattering (QLS) technique to be nearly constant. The QLS results led to a suggestion for equilibrium models of CDC aggregate formation. According to the established relationship and the suggested models, the best curve-fitting model was selected by a least-squares technique. Furthermore, the model parameters were quantified. Based on the quantified parameters, at a minimum detectable concentration of simple CDC micelles to be â¼0.2 mM, an appropriate model corresponding concentration of CDC monomers was estimated to be â¼3.08 mM. This value is consistent with a minimum monomer CDC concentration of â¼3.13 mM for simple CDC micelle formation estimated according to the present QLS detection and the model prediction. The consistency confirms the model prediction that at a low CDC monomer concentration (<3 mM), the concentration of stable CDC dimers is much higher than that of simple CDC micelles but the contribution of simple CDC micelles to the total CDC concentration cannot be negligible.
Keywords
Related Topics
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Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Yi-Shou Lin, Kai-Chi Su, Ranjith K. Kankala, Chia-Hung Lee, Chen-Lun Liu, Yu-Fang Hu,