Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8522536 | Saudi Pharmaceutical Journal | 2018 | 7 Pages |
Abstract
Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma), HeLa (cervical carcinoma), HEp-2 (larynx carcinoma) and HepG-2 (hepatocellular carcinoma) cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ± â¯0.1 to 217.0â¯Â ± â¯0.3â¯Âµg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5â¯Â±â¯0.3, 059.0â¯Â±â¯0.5 and 059.0â¯Â±â¯0.1 for HEp-2, Hela, and HepG-2 respectively). The isolated compounds (H1&H2) were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5-20.3â¯Î¼g/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells.
Related Topics
Health Sciences
Pharmacology, Toxicology and Pharmaceutical Science
Pharmaceutical Science
Authors
Fatmah A.S. Alasmary, Amani S. Awaad, Mehnaz Kamal, Saleh I. Alqasoumi, Mohamed E. Zain,