| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8533890 | Journal of Pharmacological and Toxicological Methods | 2018 | 41 Pages |
Abstract
The application of an extra top layer of collagen on the SCRH prolongs their useful life-span to 7Â days. Therefore, SCRH-plus cultures will broaden the applications of SCRH in terms of long-term toxicity evaluation and when determining metabolism of low turnover compounds.
Keywords
CDFMrp2ECMHBSSSTLCyPCDFDASandwich-cultured rat hepatocytesHEPES4-2-hydroxyethyl-1-piperazineethanesulfonic acidFBSSCRHDMEMSandwich-cultured hepatocytesPBSWEMDulbeccós modified Eaglés mediumAUCDMSOHanks balanced salt solutioncytochrome p450 enzymeSCHDimethylsulfoxidefetal bovine serumbiliary excretion indexPhosphate buffered salineExtracellular matrixarea under the curveMethodselimination rate constantBEIIntrinsic clearancemultidrug resistance-associated protein 2bile canaliculiCollagenClint
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Authors
Marlies Oorts, Janneke Keemink, Neel Deferm, Robin Adriaensen, Lysiane Richert, Patrick Augustijns, Pieter Annaert,