Phytochemical composition changes in untreated stem juice of Tinospora cordifolia (W) Mier during refrigerated storage

Initial analysis of MSn data showed presence of 14,101 molecular features in the samples. Variation in the molecular features analysed using principal component analysis and discrimination models showed 99% variation across the samples analysed on 0, 15 and 30 day. Jatrorrhizine, mangoflorine, menisperine, columbamine, berberine and tinosporoside along with other most abundant metabolites were degraded 70-80% over a period of 15 days and 99% in 30 days. High degradation rate of metabolites constituents was observed may be due to the enzymes present in the juice or oxidative reactions. Therefore, it is recommended to use the fresh juice of T. cordifolia and stability of its herbal formulation should be analysed carefully.