Chromatographic analysis of polyherbal extract and formulation by HPTLC and GC-MS methods

The present study reports the chromatographic analysis of a polyherbal extract and formulation by HPTLC and GC-MS methods. The polyherbal extract was prepared from the combined ethanolic extracts of Saraca indica, Symplocos racemosa, Hemidesmus indicus, Aloe vera, Asteracantha longifolia, Erythrina indica and Tribulus terrestris. The polyherbal extract was compressed into tablets after the addition of the required excipients. HPTLC studies revealed that there was good correlation between the extract and formulation. The HPTLC chromatogram of both extract and formulation showed Rf values at 0.03, 0.33, 0.48, 0.63 and 0.76. The phytoconstituents present in both the extract and formulation were identified by GC-MS analysis.