Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8749569 | Microbial Pathogenesis | 2018 | 18 Pages |
Abstract
Salmonella Enteritidis and Salmonella Typhimurium are among the most prevalent serotypes isolated from salmonellosis outbreaks and poultry. Salmonella spp. have the capacity to form biofilms on several surfaces, which can favour survival in hostile environments, such as slaughterhouses. Salmonella strains present differences in pathogenicity. However, there is little information regarding the pathogenicity of S. Enteritidis and S. Typhimurium isolated from avian sources and their relationship to biofilm production. The aim of this study was to use a novel pathogenicity index and a biofilm production assay to evaluate their relationships within these serotypes. In addition, we detected the presence of the spiA and agfA genes in these strains. Biofilm formation was investigated at two temperatures (37â¯Â°C and 28â¯Â°C) using microtiter plate assay, and the results were compared with the individual pathogenicity index of each strain. PCR was used to detect spiA and agfA, virulence genes associated with biofilm production. S. Enteritidis and S. Typhimurium strains were capable of producing biofilm at 37â¯Â°C and 28â¯Â°C. Sixty-two percent and 59.5% of S. Enteritidis and 73.8% and 46.2% of S. Typhimurium produced biofilm at 37â¯Â°C and 28â¯Â°C, respectively. Biofilm production at 37â¯Â°C was significantly higher in both serotypes. Only S. Enteritidis was capable of adhering strongly at both temperatures. Biofilm production was related to pathogenicity index only at 28â¯Â°C for S. Enteritidis. spiA and agfA were found in almost all strains and were not statistically associated with biofilm production.
Keywords
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Microbiology
Authors
Karen Apellanis Borges, Thales Quedi Furian, Sara Neves de Souza, Rafaela Menezes, Diane Alves de Lima, Flávia Bornancini Borges Fortes, Carlos Tadeu Pippi Salle, Hamilton Luiz Souza Moraes, Vladimir Pinheiro Nascimento,