Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8749953 | Microbial Pathogenesis | 2017 | 24 Pages |
Abstract
V. parahaemolyticus are bacteria that cause the Acute Hepatopancreatic Necrosis Disease (AHPND), or Early Mortality Syndrome (EMS), in shrimp. To further understand the pathogenesis mechanisms of V. parahaemolyticus infection in shrimp, the spreading of this bacterium in various tissues was investigated. The spread of infection in shrimp that were exposed to seawater bacteria was studied by PCR and histopathology at 1Â min, 1, 6, 12, 24, 48 and 72Â h after exposure. The PCR results showed that V. parahaemolyticus was at its most widespread at 6Â h after exposure, at which point V. parahaemolyticus was found in the gills, hepatopancreas, intestine, muscles, and hemolymph. However, examinations after 6Â h of infection found only small amounts of V. parahaemolyticus in hepatopancreas and intestines. Histopathology of the hepatopancreas showed abnormalities on gross examination at 1 min-72Â h after exposure. This study indicates that V. parahaemolyticus can spread quickly by using the hepatopancreas as the target tissue. After 6Â h of infection, V. parahaemolyticus was eliminated by immune system while their toxins still caused damage to shrimp tissues.
Keywords
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Authors
Umaporn Khimmakthong, Pimwarang Sukkarun,