| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8750048 | Microbial Pathogenesis | 2016 | 8 Pages |
Abstract
Prediction of glycosylation sites and analysis of mutant Rv0431 proteins and Rv0431 protein. A. Prediction of glycosylation sites. 59Thr, 60Thr, 61Thr, 62Thr were predicted to be glycosylated with possibility. B. SDS-PAGE of purified mutant Rv0431 proteins and purified Rv0431 protein in ÎM5447. M. PageRuler Prestained Protein Ladder (Fermentas); Lane 1. Rv0431m3/M; lane 2. Rv0431 expressed in ÎM5447; lane 3. Rv0431m1/M; lane 4. Rv0431m2/M; lane 5. Rv0431m4/M; lane 6. Rv0431/M. C. Western blot of purified mutant Rv0431 protein and Rv0431 protein in ÎM5447 using anti-His tag antibody. M. PageRuler Prestained Protein Ladder (Fermentas); Lane 1. Rv0431m3/M; lane 2. Rv0431 expressed in ÎM5447; lane 3. Rv0431m1/M; lane 4. Rv0431m2/M; lane 5. Rv0431m4/M; lane 6. Rv0431/M. D. Western blot of purified Rv0431 protein and Rv0431 protein in ÎM5447 using ConA. M. PageRuler Prestained Protein Ladder (Fermentas); Lane 1. Rv0431m3/M; lane 2. Rv0431 expressed in ÎM5447; lane 3. Rv0431m1/M; lane 4. Rv0431m2/M; lane 5. Rv0431m4/M; lane 6. Rv0431/M.140
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Authors
Guoying Deng, Fei Zhang, Shufeng Yang, Jian Kang, Shanshan Sha, Yufang Ma,