Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8792087 | Experimental Eye Research | 2018 | 62 Pages |
Abstract
The myeloid cell-specific VEGF-A knockout did not change relative retinal VEGF-A mRNA levels, the relative avascular area or macrophage/granulocyte numbers in oxygen-induced retinopathy and under normoxic conditions. We observed an insignificantly attenuated pathology in systemically clodronate-liposome treated knockouts but evident VEGF-A expression in activated Müller cells on immunohistochemically stained sections. MIO-M1 cells had significantly higher expression levels of VEGF-A in co-culture with BV-2Â cells compared to cultivating MIO-M1 cells alone. Our data show that myeloid-derived cells contribute to pathological neovascularization in oxygen-induced retinopathy through activation of VEGF-A expression in Müller cells.
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Authors
Christina Nürnberg, Norbert Kociok, Claudia Brockmann, Timo Lischke, Sergio Crespo-Garcia, Nadine Reichhart, Susanne Wolf, Ria Baumgrass, Sabine A. Eming, Sandra Beer-Hammer, Antonia M. Joussen,