Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8842840 | Research in Microbiology | 2018 | 36 Pages |
Abstract
Bacterial non-coding RNAs (ncRNAs) are widely studied and found to play important roles in regulating various cellular processes. Recently, many ncRNAs have been discovered to be transcribed or processed from 3â² untranslated regions (3â² UTRs). Here we reported a novel 3â² UTR-derived ncRNA, RibS, which could influence biofilm formation of Salmonella enterica serovar Typhi (S. Typhi). RibS was confirmed to be a â¼700Â nt processed product produced by RNase III-catalyzed cleavage from the 3â² UTR of riboflavin synthase subunit alpha mRNA, RibE. Overexpression of RibS increased the expression of the cyclopropane fatty acid synthase gene, cfa, which was located at the antisense strand. Biofilm formation of S. Typhi was enhanced by overexpressing RibS both in the wild type strain and cfa deletion mutant. Deletion of cfa attenuated biofilm formation of S. Typhi, while complementation of cfa partly restored the phenotype. Moreover, overexpressing cfa enhanced the biofilm formation of S. Typhi. In summary, RibS has been identified as a novel ncRNA derived from the 3â² UTR of RibE that promotes biofilm formation of S. Typhi, and it appears to do so, at least in part, by increasing the expression of cfa.
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Authors
Xin Zhao, Rui Liu, Hao Tang, George Osei-Adjei, Shungao Xu, Ying Zhang, Xinxiang Huang,