Key operating parameters affecting nitrogen removal rate in single-stage deammonification

The key operating parameters for improving the nitrogen removal rate (NRR) in a sequencing batch reactor (SBR) for deammonification were investigated. The major operating strategies were the coexistence between deammonification and denitrification with a carbon/nitrogen (C/N) ratio of 0.5 and the control of the number of sub-cycles based on substrate concentration for anaerobic ammonium oxidation (ANAMMOX) and ammonium oxidizing bacteria (AOB). In the study, denitrification with the addition of an organic source was beneficial for improving the NRR from 0.5 ± 0.01 kg N m−3 d−1 to 0.53 ± 0.01 kg N m−3 d−1 by removing the nitrate produced as a by-product of ANAMMOX. Unlike the gradual increase of the specific activity for AOB, the specific ANAMMOX activity (SAA) was maximized when an ammonium concentration supplied after sub-feeding phase was increased from 20 to 100 mg L−1, which increased the NRR from 0.53 ± 0.01 kg N m−3 d−1 to 0.79 ± 0.01 kg N m−3 d−1. This result suggested that the SAA is more important than the specific activity for AOB as a parameter for controlling the NRR in the single-stage deammonification. In the whole experimental period, the granule size smaller than 100 μm accounted for 52.5 ± 0.9%, making the largest contribution to the activity for AOB and denitrifiers. However, the granule size larger than 100 μm made the greatest contribution (83.8 ± 0.5%) to SAA. The feasibility of using the derivate of pH and OPR as indirect parameters to control the NRR was verified.