Advances and applications of immune receptor sequencing in systems immunology

High-throughput immunoglobulin and T cell receptor sequencing is a rapidly evolving technology that is utilized to explore the complex behavior of the adaptive immune system. Presently, advances in paired immune receptor sequencing enable the identification of hundreds of thousands of complete receptor heterodimers in a single experiment and the integration of immune receptor sequencing with transcriptional profiling. Recent studies have applied high-throughput immune receptor sequencing to understand the clonal dynamics and properties of antigen-specific B and T cells in response vaccination, autoimmunity, cancer and microbiota. A review of recent technological advances and applications of immune receptor sequencing is presented.