Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8944984 | Microbial Pathogenesis | 2018 | 11 Pages |
Abstract
The Alpinia purpurata inflorescence contains a lectin (ApuL), which has immunomodulatory activities on human cells. In the present work, it was evaluated the antibacterial and antifungal effects of ApuL against human pathogens. ApuL showed bacteriostatic activity against non-resistant (UFPEDA-02) and an oxacillin-resistant isolate (UFPEDA-672) of Staphylococcus aureus with minimal inhibitory concentrations (MIC50) of 50 and 400â¯Î¼g/mL, respectively. In addition, it showed bactericidal effect on the non-resistant isolate (minimal bactericidal concentration: 200â¯Î¼g/mL). For Candida albicans and Candida parapsilosis, ApuL showed fungistatic effect (MIC50: 200 and 400â¯Î¼g/mL, respectively). The lectin was able to impair the viability of the microorganism cells, as indicated by propidium iodide (PI) staining. Analysis of growth curves, protein leakage, and ultrastructural changes supported that ApuL acts through distinct mechanisms on S. aureus isolates. Ultrastructural analysis of ApuL-treated Candida cells revealed malformations with elongations and bulges. ApuL-oxacillin combination showed synergistic effect on the oxacillin-resistant isolates UFPEDA-670 and 671, which were not sensitive to lectin alone. Synergism was also detected for ApuL-ceftazidime against a multidrug-resistant isolate of Pseudomonas aeruginosa. Synergistic action of ApuL-fluconazole was detected for C. parapsilosis, which was insensitive to the drug alone. Biofilm formation by S. aureus non-resistant isolate and C. albicans was remarkably inhibited by ApuL at sub-inhibitory concentrations. In conclusion, ApuL showed differential effects on non-resistant and resistant bacterial isolates, was active against Candida species, and showed synergistic action in combination with antibiotics.
Keywords
CFUSabouraud dextrose agarminimal fungicidal concentrationOrSAURMMBCMHAMICSDAMFCSDBMHbmethicillin-resistant S. aureusStaphylococcus aureusMRSAOxacillinMinimal bactericidal concentrationMinimal inhibitory concentrationCeftazidimePseudomonas aeruginosaHemagglutinating activityFluconazoleSEMMueller Hinton AgarMueller Hinton BrothScanning electron microscopycolony-forming unitsPropidium iodideoptical densityCandidaPositive controlnegative control
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Authors
Gustavo Ramos Salles Ferreira, Jéssica de Santana Brito, Thamara Figueiredo Procópio, Nataly Diniz de Lima Santos, Bárbara José Rocha Cardoso de Lima, Luana Cassandra Breitenbach Barroso Coelho, Daniela Maria do Amaral Ferraz Navarro,