Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8949260 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2018 | 10 Pages |
Abstract
The histone acetyltransferase HBO1 (Histone acetyltransferase binding to origin recognition complex 1, Myst2/Kat7) participates in a range of life processes including DNA replication and tumorigenesis. Recent studies revealed that HBO1 is involved in gene transcriptional activation. However, the molecular behavior of HBO1 in inflammation is yet to be studied. Here we report that endotoxin lipopolysaccharide (LPS) elevates HBO1 protein level via up-regulating UPS25 (ubiquitin specific peptidase 25) and alters inflammatory gene transcription in THP-1 monocytes and in human primary macrophages. LPS protects HBO1 from ubiquitin proteasomal degradation without significantly altering its transcription. By immunoprecipitation, we identified that HBO1 associates with a deubiquitinating enzyme USP25 in THP-1 cells. LPS increases protein level of USP25 resulting in accumulation of HBO1 by suppression of HBO1 ubiquitination. Stabilized-HBO1 modulates inflammatory gene transcription in THP-1 cells. These findings indicate that USP25 promotes stability of HBO1 in bacterial infection thereby enhances HBO1-mediated inflammatory gene transcription.
Keywords
LPSDUBSIX1Histone H3 lysine 27 trimethylationH3K4me3H3K27me3MCMCDK2Minichromosome maintenance complexCdt1HDAC11TLR4SCFDsiRNACHXHBO1MYD88TRIFdeubiquitinating enzymeinflammationmyeloid differentiation primary response 88TIR-domain-containing adapter-inducing interferon-βGene transcriptioncycloheximidelipopolysaccharideToll like receptor 4histone H3 lysine 4 trimethylationcyclin dependent kinase 2ubiquitination
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Chen Long, Yandong Lai, Jin Li, Jiangsheng Huang, Chunbin Zou,