Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8955945 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Receptor-like protein kinase (RLKs) plays pivotal roles in plant growth and development as well as stress responses. However, little is known about the function of RLKs in Nitotiana tobacum. In the present study, we present data on NtRLK5, a novel RLK-like gene isolated from Hongda (Nitotiana tobacum L.). Expression profile analysis revealed that NtRLK5 was strongly induced by drought and salt stresses. Transient expression of NtRLK5-GFP fusion protein in protoplast showed that NtRLK5 was localized to plasma membrane. Overexpression of NtRLK5 conferred enhanced drought tolerance in transgenic Arabidopsis plants, which was attributed to not only the lower malondialdehyde (MDA) and H2O2 contents, but also the higher antioxidant enzymes activities. Moreover, the expression of several antioxidation- and stress-related genes was also significantly up-regulated in NtRLK5 transgenic plants under drought condition. Taken together, the results suggest that NtRLK5 functions as a positive regulator in drought tolerance.
Keywords
ABARLKRLKsMDAPODRELCATqRT-PCRGFPMurashige and Skoog mediumO2−RNA interferenceRNAiROSHydrogen peroxideArabidopsisabscisic acidDrought toleranceSODSuperoxide dismutasemalondialdehydeRelative electrolyte leakagewild typeH2O2quantitative real-time PCRPeroxidasegreen fluorescent proteinReceptor-like protein kinasepolyethylene glycolPEGCatalaseReactive oxygen species
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Authors
Guoyun Xu, Mingjuan Li, Hui Zhang, Qiansi Chen, Lifeng Jin, Qingxia Zheng, Pingping Liu, Peijian Cao, Xia Chen, Niu Zhai, Huina Zhou,