| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8955946 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Retroviruses have two essential activities: reverse transcription and integration. The viral protein integrase (IN) covalently joins the viral cDNA genome to the host DNA. Prototype foamy virus (PFV) IN has become a model of retroviral intasome structure. However, this retroviral IN has not been well-characterized biochemically. Here we compare PFV IN to previously reported HIV-1 IN activities and discover significant differences. PFV IN is able to utilize the divalent cation calcium during strand transfer while HIV-1 IN is not. HIV-1 IN was shown to completely commit to a target DNA within 1â¯min, while PFV IN is not fully committed after 60â¯min. These results suggest that PFV IN is more promiscuous compared to HIV-1 IN in terms of divalent cation and target commitment.
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Authors
R.M. Mackler, M.A. Lopez, M.J. Osterhage, K.E. Yoder,