Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8955965 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Liver-X-receptors (LXRs) are ligand-activated transcription factors belonging to the nuclear receptor superfamily. The two popular homologous receptor subtypes, LXRα and LXRβ, exhibit differential expression patterns, thereby probably playing different roles in different contexts. This study aimed to evaluate the different roles of the two LXR subtypes and the mechanisms underlying their protection of cardiomyocytes against high-glucose stress. Silencing of LXRα, but not LXRβ impaired normal LXR-mediated cardioprotective effects against high glucose-induced oxidative stress, apoptosis, and inflammation. Mechanistically, silencing of small ubiquitin-like modifier (SUMO)1 or SUMO2/3 did not affect LXR-mediated cardioprotective effects; however, these were impaired in response to nuclear receptor corepressor (NCoR) silencing. Together, these findings indicate that LXRα, but not LXRβ, protects against high glucose-induced cardiomyocyte injury, probably via the NCoR-dependent transrepression of downstream target genes.
Keywords
RT-qPCRSUMODCMRNScyt-cNCoRLXRFBSDMEMliver-X-receptoriNOSPBSNF-κBGAPDHDulbecco's modified Eagle's mediumROSExpressionSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisanalysis of varianceANOVAOxidative stressstandard error of the meanDiabetes mellitusreverse transcription quantitative polymerase chain reactionfetal bovine seruminducible nitric oxide synthasecytochrome cCardiomyocytesPhosphate-buffered salineSEMnuclear receptor corepressorPropidium iodidediabetic cardiomyopathysmall ubiquitin-like modifierGlucosehigh glucoseglyceraldehyde-3-phosphate dehydrogenasereactive nitrogen speciesReactive oxygen species
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Qing He, Fengdan Wang, Yuqi Fan, Changqian Wang, Junfeng Zhang,