Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8961865 | Biochemical and Biophysical Research Communications | 2018 | 6 Pages |
Abstract
Mice underwent bilateral renal IRI for 26â¯min or sham surgery. After surgical procedure, animals were injected either with hemin (5â¯mg/kg) or vehicle. Twenty-four hours later, mice were sacrificed. Despite strong HO-1 induction, hemin-treated mice exhibited significant renal damage and oxidative stress as compared to vehicle-treated mice. Interestingly, higher dose of hemin is associated with more severe IRI-induced AKI in a dose-dependent relation. To determine whether hemin preconditioning remains efficient to dampen postoperative hemin-amplified IRI-induced AKI, we pretreated mice either with hemin (5â¯mg/kg) or vehicle 24â¯h prior to surgical procedure. Then, all mice (hemin- and vehicle-pretreated) received postoperative injection of hemin (5â¯mg/kg) to amplify IRI-induced AKI. In comparison to vehicle, prior administration of hemin to renal IRI mitigated hemin-amplified IRI-induced AKI as attested by fewer renal damage, inflammation and oxidative stress. In conclusion, hemin may have a dual effect on renal IRI, protective or deleterious, depending on the timing of its administration.
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Authors
Maxime Rossi, Sandrine Delbauve, Eric Wespes, Thierry Roumeguère, Oberdan Leo, Véronique Flamand, Alain Le Moine, Jean-Michel Hougardy,