Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8993459 | Il Farmaco | 2005 | 4 Pages |
Abstract
A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for quantification of amlodipine in plasma. The assay enables the measurement of amlodipine for therapeutic drug monitoring with a minimum detectable limit of 0.2 ng mlâ1. The method involves simple, one-step extraction procedure and analytical recovery was about 97%. The separation was performed on an analytical 125 Ã 4.6 mm i.d. Nucleosil C8 column. The wavelength was set at 239 nm. The mobile phase was a mixture of 0.01 M sodium dihydrogen phosphate buffer and acetonitrile (63:37, v/v) adjusted to pH 3.5 at a flow rate of 1.5 ml min-1. The calibration curve was linear over the concentration range 0.5-16 ng mlâ1. The coefficients of variation for inter-day and intra-day assay were found to be less than 10%.
Keywords
Related Topics
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Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
A. Zarghi, S.M. Foroutan, A. Shafaati, A. Khoddam,