2,2'-Bipyridine as a new and sensitive spectrophotometric reagent for the determination of nanoamounts of certain dibenzazepine class of tricyclic antidepressant drugs

2,2'-bipyridine is proposed as new and sensitive spectrophotometric reagent for the determination of certain dibenzazepine class of tricyclic antidepressants. The spectrophotometric method is based on the reaction of imipramine hydrochloride (IPH), desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH), trimipramine maleate (TPM) and opipramol (OPP) with iron (III) and subsequent reaction with 2,2'-bipyridine in an acetic acid medium to yield a pink color with maximum absorption at 530 nm. The color developed was stable over 3-4 h at room temperature (~27 oC). The commonly encountered excipients and additives did not interfere with the determination. Results from the analysis of pure drugs and commercial tablets agreed well with those of the official method (United States Pharmacopoeia, 24, USP Convention, Rockville 2000, pp. 505-506, 865-867.).