Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9001735 | Biochemical Pharmacology | 2005 | 9 Pages |
Abstract
The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube formation was related to α6 integrin subunit expression, which was enhanced by 50% in the presence of the growth factors. Indeed, a monoclonal anti-α6 blocking antibody abolished this vascular tube formation. EPS had no effect in any of the experimental conditions, underlying the importance of sulfation in the angiogenic effects of exopolysaccharide. By potentiating the angiogenic activity of FGF-2 and/or VEGF, OS-EPS, which possesses low anticoagulant activity and thus a low hemorrhagic risk, could potentially be used to accelerate vascular wound healing or to promote the growth of collateral blood vessels in ischemic tissues.
Keywords
LMWAngiogenic growth factorHSPGFGF-2HMWHUVECEPSFBSAngiogenesisExopolysaccharidefetal bovine serumHuman umbilical vein endothelial cellVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)fibroblast growth factor-2Matrigelhigh molecular weightlow molecular weightheparan sulfate proteoglycansPolysaccharideCollagen gel
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Authors
Sabine Matou, Sylvia Colliec-Jouault, Isabelle Galy-Fauroux, Jacqueline Ratiskol, Corinne Sinquin, Jean Guezennec, Anne-Marie Fischer, Dominique Helley,