Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9002071 | Biochemical Pharmacology | 2005 | 7 Pages |
Abstract
Quercetin 3-O-β-(2â³-galloyl)-rhamnopyranoside (QGR) is a naturally occurring quercitrin gallate, a polyphenolic compound isolated from Persicaria lapathifolia (Polygonaceae). In the present study, QGR compound was discovered to have inhibitory effect on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7. QGR compound attenuated LPS-induced synthesis of both mRNA and protein of inducible nitric oxide synthase (iNOS), in parallel, and inhibited LPS-induced luciferase expression as a reporter of iNOS promoter activity in the macrophages. As a mechanism of the anti-inflammatory action shown by QGR compound, suppression of nuclear factor (NF)-κB activation has been documented. QGR compound exhibited inhibitory effect on LPS-mediated NF-κB transcriptional activity in macrophages RAW 264.7. Furthermore, the compound inhibited LPS-mediated nuclear translocation of NF-κB p65 and DNA binding activity of NF-κB complex, in parallel, but did not influence LPS-mediated IκBα degradation. Taken together, QGR compound suppressed LPS-mediated NF-κB activation, specifically to nuclear localization step of NF-κB p65, which was attributable to its down-regulatory action on LPS-induced NO production and iNOS expression.
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Authors
Byung Hak Kim, Sung Min Cho, Alavala Matta Reddy, Yeong Shik Kim, Kyung Rak Min, Youngsoo Kim,