Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9194539 | Journal of Neuroimmunology | 2005 | 9 Pages |
Abstract
Demyelinating polyneuropathiy associated with IgM paraproteinemia and high titers of anti-MAG IgM antibodies (MAG-PN) is considered different from chronic inflammatory demyelinating polyneuropathy, particularly because of the poorer response to treatment of MAG-PN patients. Therefore, anti-MAG anitbodies may have relevant prognostic value. Available anti-MAG antibody assays require central nervous system myelin proteins from autopsied human brains. This study investigated the feasibility of detecting anti-MAG antibody by immunofluorescence and flow cytometry using a panel of human neuroblastoma cell lines as targets. We report here on the evaluation of the LA-N-1 cell line as an appropriate substrate for the detection of anti-MAG antibody by indirect immunoflourescence.
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Authors
Gianluca Isoardo, Silvia Deaglio, Dario Cocito, Giuseppe Migliaretti, Enza Ferrero, Franco Cavallo, Luca Durelli, Fabio Malavasi,