| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9268259 | Journal of Clinical Virology | 2005 | 4 Pages |
Abstract
This study was designed to assess the effect of sequence mismatches between primers and their targets on viral quantitative PCR. Numerous primers were constructed incorporating various mismatches with a target sequence on the BKV T antigen gene. When using these primers in standard Taqman two-step cycling conditions, as few as two mismatches in a single primer increased cycle threshold values and significantly influenced the calculation of viral load.
Keywords
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Authors
David M. Whiley, Theo P. Sloots,