Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9275423 | Clinical Microbiology and Infection | 2005 | 5 Pages |
Abstract
A strain of Klebsiella pneumoniae resistant to cefoxitin and oxyimino-cephalosporins, but susceptible to cefepime, was isolated from an adult patient hospitalised in Taichung, Taiwan. Isoelectric focusing revealed three β-lactamases with isoelectric points of 5.4, 8.2 and 7.9, respectively. Following PCR with plasmid DNA templates and gene sequencing, these enzymes were shown to correspond to TEM-1, SHV-5 and a novel DHA-1-like enzyme (designated DHA-3). The bla genes for TEM-1 and SHV-5 were transferable, but the bladha-3 gene was non-self-transferable in conjugation experiments. All three bla genes were successfully introduced by electrotransformation into an Escherichia coli recipient (DH5α), resulting in a similar resistance profile to that observed in the original donor strain. Other K. pneumoniae strains producing DHA-1-like enzymes have been identified previously in Taiwan, and this report suggests that DHA-type β-lactamases are continuing to emerge in this country.
Related Topics
Life Sciences
Immunology and Microbiology
Microbiology
Authors
L.-T. Wu, S.-W. Hung, Y.-C. Chuang, H.-E. Chen, R.N. Jones, W.-L. Yu,