Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9341441 | Experimental Eye Research | 2005 | 6 Pages |
Abstract
To facilitate the peeling of internal limiting membrane or epiretinal membrane in vitreoretinal surgery, trypan blue (TB) and indocyanine green (ICG) have been used. However, the cytotoxicity of these dyes have been concerned. The aim of this study was to investigate the neurotoxic effects of TB on rat retinal ganglion cells (RGCs) and compare the effect of TB with ICG. Rat RGCs were purified by a two-step immunopanning procedure. In short-time exposure experiments, purified RGCs cultured for 3 days were exposed to 600 mg Lâ1 TB, 1500 mg Lâ1 TB and 1500 mg Lâ1 ICG for 10 sec to 30 min. The number of viable RGCs was counted after 12 hr in culture. In long-time exposure experiments, purified RGCs were cultured for 3 days in TB solutions ranging from 2 to 800 mg Lâ1 or in ICG solutions at concentrations from 2 to 250 mg Lâ1. Then the number of viable cells was counted. Exposure to 600 mg Lâ1 TB, 1500 mg Lâ1 TB and 1500 mg Lâ1 ICG for short time from 10 sec to 30 min caused a time-dependent damage to RGCs. There was no significant difference in cytotoxicity to RGCs between TB and ICG in short-time exposure. In long-time exposure, TB as well as ICG showed neurotoxic effect on RGCs in a dose-dependent manner. Fifty percentage inhibitory concentration (IC50) of TB to RGCs was calculated as 115 mg Lâ1, while that of ICG was 33 mg Lâ1. In conclusion, TB induced neurotoxic effect on RGCs in a dose- and time-dependent manner. During the short-time exposure in surgery, there is likely no difference in neurotoxic effect on RGCs between TB and ICG. However, once the dyes are left in the eyes after surgery, TB may show less toxicity on RGCs than ICG.
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Authors
Yan Jin, Saiko Uchida, Yasuo Yanagi, Makoto Aihara, Makoto Araie,