| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9602408 | Biomolecular Engineering | 2005 | 9 Pages |
Abstract
This review deals with new mutagenesis and recombination strategies published recently. A full overview of new methods for creating more specialised mutant libraries is given. The importance of selection in directed evolution strategies is being exemplified by some current successes including the β-lactam acylases.
Keywords
FACSAATaseepPCRSESAMIVCDHRFSSNRRmAbsADOLBDMMPHTSDBDGFPAspartate aminotransferaseSelectionDirected evolutionTyrosine aminotransferaseerror-prone polymerase chain reactionDNA binding domainligand binding domainRecombinationNew methodshigh throughput screeningfluorescence activated cell sortingMatrix metalloproteaseMutagenesisgreen fluorescent protein
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Linda G. Otten, Wim J. Quax,