Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9603109 | Journal of Bioscience and Bioengineering | 2005 | 7 Pages |
Abstract
We describe the successful heterologous expression of the Solanum tuberosum α-glucan phosphorylase (GP) gene in Aspergillus niger. Special attention was paid to the influence of different codon usage and A+T content in the coding region on GP protein expression. Use of A. niger-preferred codon usage and lower A+T content in a synthetic gene (GP-syn) resulted in a significant improvement in the level of the GP mRNA and a dramatic increase in the quantity of GP protein produced such that it accounted for approximately 10% of the total soluble protein. We suggest that redesigning the primary DNA sequence encoding a desired protein product can be an extremely effective method for improving heterologous protein production in filamentous fungi.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Akio Koda, Takayuki Bogaki, Toshitaka Minetoki, Masato Hirotsune,