Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9603157 | Journal of Bioscience and Bioengineering | 2005 | 4 Pages |
Abstract
The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermoanaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens α-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1 g of KP and 4.9 g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selaginose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Takuo Yamamoto, Kazuhisa Mukai, Kazuhiko Maruta, Hikaru Watanabe, Hiroshi Yamashita, Tomoyuki Nishimoto, Michio Kubota, Hiroto Chaen, Shigeharu Fukuda,