Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9603223 | Journal of Bioscience and Bioengineering | 2005 | 5 Pages |
Abstract
GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of OLE1, which encodes a Î-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using OLE1 promoter:lacZ fusion reporter genes revealed that both the cis element of the OLE1 promoter and trans factors are involved in the increased expression of OLE1 in sake yeasts.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Tasuku Yamada, Hitoshi Shimoi, Kiyoshi Ito,