Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9603274 | Journal of Bioscience and Bioengineering | 2005 | 4 Pages |
Abstract
Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobacterium tumefaciens with a binary expression vector. Using the improved transformation method, we introduced a major allergen gene from a house dust mite, Der f 1, into MG-20. Analyses by Southern hybridization, reverse transcription (RT)-PCR, and Western blotting showed that the Der f 1 gene was integrated into the genome of L. japonicus, expressing the gene product in the T1 lines. Our results imply future application of oral allergen-specific immunotherapy using legume plants.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Tomoaki Kato, Yuji Goto, Kazuhisa Ono, Makoto Hayashi, Yoshikatsu Murooka,