Article ID Journal Published Year Pages File Type
9603274 Journal of Bioscience and Bioengineering 2005 4 Pages PDF
Abstract
Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobacterium tumefaciens with a binary expression vector. Using the improved transformation method, we introduced a major allergen gene from a house dust mite, Der f 1, into MG-20. Analyses by Southern hybridization, reverse transcription (RT)-PCR, and Western blotting showed that the Der f 1 gene was integrated into the genome of L. japonicus, expressing the gene product in the T1 lines. Our results imply future application of oral allergen-specific immunotherapy using legume plants.
Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
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