Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604240 | Journal of Biotechnology | 2005 | 7 Pages |
Abstract
A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100Â pg/ml. The minimum streptavidin detection limit using this technique is 1Â pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.
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Authors
Yosuke Amemiya, Tsuyoshi Tanaka, Brandon Yoza, Tadashi Matsunaga,