Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604255 | Journal of Biotechnology | 2005 | 13 Pages |
Abstract
In total, 33 promoters were selected which cover a broad range of promoter activities and induction times indicating that the strength of promoters can be modulated by partially randomizing the sequence upstream of the â10 region. The induction ratio of synthetic promoters at the transition from exponential to stationary-phase was from 4 to over 6000 and the induction time relative to the entrance into stationary-phase from â1.4 to 2.7Â h. Ninety-one percentage of the promoters had no or only low background activity during exponential growth. The broad variability of the promoters offers good possibilities for fine-tuning of gene expression and for applications in industrial bioprocesses.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Gerhard Miksch, Frank Bettenworth, Karl Friehs, Erwin Flaschel, Axel Saalbach, Thorsten Twellmann, Tim W. Nattkemper,