Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604324 | Journal of Biotechnology | 2005 | 10 Pages |
Abstract
Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids β-(1,4) linked and variably C2 and/or C3O-acetylated. To obtain β-Î-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using 1H NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan.
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Bioengineering
Authors
C. Delattre, P. Michaud, J.M. Lion, B. Courtois, J. Courtois,