Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604342 | Journal of Biotechnology | 2005 | 8 Pages |
Abstract
To analyze the functionality of alternative 3â²-UTR processing in the yeast Kluyveromyces lactis, recombinant forms of the KlCYC1 gene containing the proximal (1-713) or the distal (699-1194) 3â²-UTR region (positions related to the TAA stop codon) were obtained. The cells expressing the gene with proximal 3â²-UTR showed the same growth phenotype as the wild type. When the gene expressed only the distal region, a single transcript was generated and its expression was increased in late-growth phases. Cells expressing the alternative distal 3â²-UTR region showed differences in their levels of cytochrome c biomass and ethanol production with respect to the wild type. The split 3â²-UTR regions were also functional as separate processing units in Saccharomyces cerevisiae. The importance of our results in recombinant gene expression applications will be discussed.
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Authors
Silvia Seoane, Bernard Guiard, Ana-MarÃa RodrÃguez-Torres, MarÃa Angeles Freire-Picos,