Article ID Journal Published Year Pages File Type
9604342 Journal of Biotechnology 2005 8 Pages PDF
Abstract
To analyze the functionality of alternative 3′-UTR processing in the yeast Kluyveromyces lactis, recombinant forms of the KlCYC1 gene containing the proximal (1-713) or the distal (699-1194) 3′-UTR region (positions related to the TAA stop codon) were obtained. The cells expressing the gene with proximal 3′-UTR showed the same growth phenotype as the wild type. When the gene expressed only the distal region, a single transcript was generated and its expression was increased in late-growth phases. Cells expressing the alternative distal 3′-UTR region showed differences in their levels of cytochrome c biomass and ethanol production with respect to the wild type. The split 3′-UTR regions were also functional as separate processing units in Saccharomyces cerevisiae. The importance of our results in recombinant gene expression applications will be discussed.
Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
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