| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9604405 | Journal of Biotechnology | 2005 | 16 Pages |
Abstract
The oligosaccharides from all animals were a mixture of high mannose-, hybrid- and complex-type oligosaccharides. Sialic acid was present as α-2,6-linkage and no α-1,3-linked galactose was observed. These results indicate that transgenic animals with closely related genetic backgrounds express recombinant protein with comparable glycosylation.
Keywords
Na2GalNAcN-acetylgalactosamineGlcNAcHPAECFucoseFucPNGase FNeuAcNeuGcHigh pH anion exchange chromatographyantithrombinN-acetylneuraminic acidN-glycolylneuraminic acidChoChinese Hamster OvaryPulsed amperometric detectiontransgenic animalsMALDI-TOF-MSMannoseManN-acetylglucosaminePADGenetic polymorphismpeptide N-glycosidase FCarbohydrateshigh-performance liquid chromatographyHPLCGalGalactose
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Qun Zhou, Josephine Kyazike, Yann Echelard, Harry M. Meade, Elizabeth Higgins, Edward S. Cole, Tim Edmunds,