Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604413 | Journal of Biotechnology | 2005 | 15 Pages |
Abstract
An important number of heterologous proteins have been produced in the methylotrophic yeast Pichia pastoris using the alcohol oxidase promoter. Two factors that drastically influence protein production and cultivation process development in this system are gene dosage and methanol assimilation capacity of the host strain (Mut phenotype). Using a battery of four strains which secrete a Rhizopus oryzae lipase (ROL), the combined effects of gene dosage and Mut phenotype on recombinant protein production in Pichia pastoris was studied in fed-batch cultures. Regarding the effect of phenotype, the specific productivity and the YP/X were 1.29- and 2.34-fold higher for MutsROL single copy strain than for Mut+ROL single copy strain. On the contrary, the productivity of Mut+ROL single copy strain was 1.34-fold higher than MutsROL single copy strain. An increase in ROL gene dosage seems to negatively affect cell's performance in bioreactor cultures, particularly in Muts strains. Overall, the Muts strain may be still advantageous to use because it allows for easier process control strategies.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Oriol Cos, Alicia Serrano, José Luis Montesinos, Pau Ferrer, James M. Cregg, Francisco Valero,