Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604429 | Journal of Biotechnology | 2005 | 6 Pages |
Abstract
We describe a novel method of PCR-mediated mutagenesis employing DNA containing a natural abasic site and translesional Taq DNA polymerase. This method incorporated an adenine (80.8%) or guanine (7.7%) residue or led to a base deletion mutation (11.2%) opposite the abasic site. We conclude that the combination of DNA containing an abasic site and translesional Taq DNA polymerase is an easy and useful technique for PCR-mediated mutagenesis, having advantages different from those of conventional error-prone PCR.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Atsushi Kobayashi, Motomitsu Kitaoka, Kiyoshi Hayashi,