Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9604461 | Journal of Biotechnology | 2005 | 9 Pages |
Abstract
We studied in batch assays the transformation and toxicity of anthraquinone dyes during incubations with anaerobic granular sludge under mesophilic (30 °C) and thermophilic (55 °C) conditions. Additionally, the electron shuttling capacity of the redox mediator anthraquinone-2-sulfonic acid (AQS) and subsequent increase on decolourisation rates was investigated on anthraquinone dyes. Compared with incubations at 30 °C, serum bottles at 55 °C presented distinctly higher decolourisation rates not only with an industrial wastewater containing anthraquinone dyes, but also with model compounds. Compared with batch assays at 30 °C, the first-order rate constant “k” of the Reactive Blue 5 (RB5) was enhanced 11-fold and 6-fold for bottles at 55 °C supplemented and free of AQS, respectively. However, the anthraquinone dye Reactive Blue 19 (RB19) demonstrated a very strong toxic effect on volatile fatty acids (VFA) degradation and methanogenesis at both 30 °C and 55 °C. The apparent inhibitory concentrations of RB19 exerting 50% reduction in methanogenic activity (IC50-value) were 55 mg lâ1 at 30 °C and 45 mg lâ1 at 55 °C. Further experiments at both temperatures revealed that RB19 was mainly toxic to methanogens, because the glucose oxidizers including acetogens, propionate-forming, butyrate-forming and ethanol-forming microorganisms were not affected by the dye toxicity.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
André B. dos Santos, Iemke A.E. Bisschops, Francisco J. Cervantes, Jules B. van Lier,