Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9607568 | Journal of Photochemistry and Photobiology C: Photochemistry Reviews | 2005 | 15 Pages |
Abstract
We have demonstrated a new concept in the design of base-discriminating fluorescent (BDF) nucleosides that can clearly distinguish the type of base opposite the BDF base by a fluorescence change. The probe DNA containing BDF nucleosides exhibits a drastic change in fluorescence intensity only when it hybridizes with a specific target sequence. This clear change in fluorescence is used for SNP typing of genes. The homogeneous SNP typing method using BDF probes would be a powerful alternative to conventional SNP typing methods. In this review, the fluorescence properties of several BDF nucleosides are described.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Akimitsu Okamoto, Yoshio Saito, Isao Saito,