Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9607665 | Process Biochemistry | 2005 | 9 Pages |
Abstract
The quantitative effects of fermentation time, temperature and broth content on mycelial growth in terms of dry cell weight (DCW) and extracellular polysaccharide content (EPC) produced by the fungus Boletus spp. ACCC 50328 in submerged fermentation were investigated separately using response surface methodology (RSM). The three factors chosen for the present investigation were based on the results of a previous Plackett-Burman (PB) design. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. By solving the regression equation and also by analyzing the response surface contour plots, the optimal batch fermentation conditions were determined: under conditions of fermentation time, 10.7 days; temperature, 24.23 °C; and broth content, 50.0 ml, EPC reached 1053.987 μg mlâ1 of fermentation liquor. When fermentation time, temperature and broth content were 10.9 days, 26.77 °C and 64.9 ml, respectively, DCW reached 4.216 mg mlâ1. In order to obtain simultaneously the maximum yield of EPC and DCW, the above conditions would be 10.5 days, 26.23 °C and 58.70 ml; and under these conditions, the maximum predicted yield of EPC and DCW were 992.258 μg mlâ1 and 4.163 mg mlâ1, respectively. These predicted values were also verified by validation experiments.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yun-Xiang Wang, Zhao-Xin Lu,