Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9607709 | Process Biochemistry | 2005 | 7 Pages |
Abstract
The effects of medium components on laccase, manganese peroxidase and glyoxal oxidase production by Trametes trogii (MYA 28-11) have been studied using Plackett-Burman (PB) experimental design. Copper had the highest positive influence on ligninolytic enzyme production. T. trogii extracellular fluids obtained in the medium which rendered the highest ligninolytic production (45.32 U/ml laccase, 214.5 mU/ml manganese peroxidase and 116 mU/ml glyoxal oxidase) also showed the greatest ability to decolorize the dyes Ponceau 2R (a xylidine derivative), malachite green and anthraquinone blue (at rates of 2.14, 1.35 μg and 3 mg dye/(ml h), respectively). The relationship among decolorization rates and ligninolytic enzyme activities was analyzed by multiple regression. The results fit a linear plus interactions model. The comparison of the response surfaces obtained, suggests that while laccase activity has a greater importance in xylidine degradation, manganese peroxidase activity plays the major role in malachite green decolorization.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
L. Levin, F. Forchiassin, A. Viale,