Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9616728 | Journal of Molecular Catalysis B: Enzymatic | 2005 | 7 Pages |
Abstract
A bienzymatic supramolecular nanoassembly containing catalase and Cu, Zn-superoxide dismutase is reported. Catalase was hydrophobically modified with 1-adamantanecarboxylic acid and then immobilized on β-cyclodextrin-coated gold nanospheres via supramolecular associations. The bienzymatic nanocatalyst was further prepared by co-immobilization of β-cyclodextrin-modified superoxide dismutase. Supramolecularly immobilized catalase and superoxide dismutase retained 73 and 35%, respectively, of their initial specific activity. The range of optimum pH for catalase was increased from 7.0-7.5 to 6.5-7.5, and the thermal stability improved by 7 °C after co-immobilization. Superoxide dismutase was 90-fold more resistant to inactivation by 100 mM H2O2 after bienzymatic immobilization with catalase on metal nanoparticles.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Reynaldo Villalonga, Roberto Cao, Alex Fragoso, Angelo E. Damiao, Pedro D. Ortiz, Julio Caballero,