Article ID Journal Published Year Pages File Type
9616751 Journal of Molecular Catalysis B: Enzymatic 2005 4 Pages PDF
Abstract
During the oxidative self-inactivation of cytochrome c, four molecular events were detected and kinetically characterized; heme destruction, loss of the iron-sulfur coordination, protein multimerization, and protein inactivation. Electron transfer balance from the four molecular events account for 100% of the electrons transferred in the first minute of protein incubation with hydrogen peroxide. However, an unidentified electron source started donating electrons after the first minute and reaches 23% of the transferred electrons after 10 min incubation. Thus, the electron transfer kinetics and its balance allowed us to detect a non-considered electron transfer and free radical formation.
Related Topics
Physical Sciences and Engineering Chemical Engineering Catalysis
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