Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9616751 | Journal of Molecular Catalysis B: Enzymatic | 2005 | 4 Pages |
Abstract
During the oxidative self-inactivation of cytochrome c, four molecular events were detected and kinetically characterized; heme destruction, loss of the iron-sulfur coordination, protein multimerization, and protein inactivation. Electron transfer balance from the four molecular events account for 100% of the electrons transferred in the first minute of protein incubation with hydrogen peroxide. However, an unidentified electron source started donating electrons after the first minute and reaches 23% of the transferred electrons after 10Â min incubation. Thus, the electron transfer kinetics and its balance allowed us to detect a non-considered electron transfer and free radical formation.
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Authors
Brenda Valderrama, Rafael Vazquez-Duhalt,