Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9616766 | Journal of Molecular Catalysis B: Enzymatic | 2005 | 7 Pages |
Abstract
The efficacy of polyoxyethylene sorbitan trioleate (Tween 85) addition on the activity of Mucor javanicus lipase was investigated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT) microemulsion-based organogels (MBGs). Gelatin was used as the gelling component of the MBGs. The maximal reaction rate was obtained at an AOT:Tween 85 molar ratio of 10:1. Under a fixed molar ratio system of AOT:Tween 85Â =Â 10:1, the reaction rate also attained a maximum at a WG (=[H2O]/[AOTÂ +Â Tween 85] in MBG phase) value of 100 and an AOT concentration of 150Â mM. The reaction proceeded under a reaction-controlled regime, and the reaction rate for the AOT/Tween 85 mixed system was about 2-fold higher than that for the AOT single system. The lipase activity was well maintained for 10 days and recovered by contacting the MBGs with concentrated amphiphile solutions.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Kazuhito Nagayama, Masanao Imai,