Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9616796 | Journal of Molecular Catalysis B: Enzymatic | 2005 | 6 Pages |
Abstract
Three genes respectively encoding d-specific hydantoinase (DHHase), N-carbamoyl-d-amino acid amidohydrolase (DCHase) and hydantoin racemase (HRase) were co-expressed in E. coli in a system designed for the efficient enzymatic production of d-amino acids via a combination of hydantoin hydrolysis and hydantoin racemization. With the use of whole cells, the d-forms of eight amino acids - d-phenylalanine, d-tyrosine, d-tryptophan, O-benzyl-d-serine, d-valine, d-norvaline, d-leucine and d-norleucine - were efficiently converted from the corresponding dl-5-monosubtituted hydantoin compounds.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Hiroyuki Nozaki, Yasuhiro Takenaka, Ikuo Kira, Kunihiko Watanabe, Kenzo Yokozeki,