Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9675562 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2005 | 5 Pages |
Abstract
In this work, we experimentally determine the translational diffusion coefficient Dt of charged polypeptides, poly-l-lysine (PLL) molecules, that change conformation from random-coil to α-helix or β-sheet upon increasing the solvent pH and temperature, respectively. We measure the scattered light component having the same polarization direction as the incident light, to construct the field autocorrelation function and obtain Dt, further estimating the PLL dimensions in the random-coil and α-helix structures. We use a commercial high molecular weight PLL sample that is widely used in many practical applications.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
L. RodrÃguez-Maldonado, A. Fernández-Nieves, A. Fernández-Barbero,