| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9675960 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2005 | 5 Pages |
Abstract
Au/Ag core-shell nanoparticles labeled by monoclonal antibody and surface-enhanced Raman scattering (SERS) probes were used for immunoassay analysis. Mouse polyclonal antibodies against hepatitis B surface antigen (mouse Anti-HBsAg PAb) were covalently immobilized on the silicon chips modified by a self-assembled monolayer of (3-amino-propyl) trimethoxysilane via glutaraldehyde (GA) activation. The Au/Ag core-shell nanoparticles were labeled by the mouse monoclonal antibody against hepatitis B surface antigen (mouse Anti-HBsAg MAb) and the probe molecule, p-mercaptobenzoic acid (MBA). Immunoreactions between the labeled Au/Ag core-shell nanoparticles and the corresponding antigens captured by the Anti-HBsAg PAb on the silicon chips were investigated by SERS.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Xiaohui Ji, Shuping Xu, Lianying Wang, Min Liu, Kai Pan, Hang Yuan, Lan Ma, Weiqing Xu, Jinghong Li, Yubai Bai, Tiejin Li,