Article ID Journal Published Year Pages File Type
9676014 Colloids and Surfaces A: Physicochemical and Engineering Aspects 2005 6 Pages PDF
Abstract
Artemisinin is an important antimalarial drug. The interaction of artemisinin with either hemin or hemoglobin (Hb) may be helpful in understanding the antimalarial mechanism of artemisinin. In the present study, the layer-by-layer films of hemoglobin/poly(vinyl sulfonate) (PVS) were grown on glassy carbon (GC) electrode and silver (Ag) electrode. The atomic force microscopy (AFM), UV-vis and fluorescence spectroscopy were used to characterize the formation of hemoglobin in layer-by-layer films. The (PVS/Hb)n films were applied to investigate the interaction of artemisinin and hemoglobin by electrochemical method. The results showed that the hemoglobin in (PVS/Hb)n films could catalyze the reduction of artemisinin by lowering the overpotential about 210 and 70 mV for GC and Ag electrodes, respectively. The catalytic reduction by the hemoglobin was compared to that by hemin. It suggested that the prosthetic group heme in Hb might induce cleavage of the peroxide bond in artemisinin.
Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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